Natural products which are capable of reducing the activities of cholinergic and metabolic enzymes are safer pest-controlling alternatives to synthetic ones. This study compared the ability of Blighia sapida stem-bark extract and Rambo, (a synthetic pesticide), to interfere with acetylcholinesterase (AChE), glutathione S-transferase (GST), and biomarkers in the brain, liver, and blood of Wistar rat. The brain and liver of rats were excised, and blood was collected into heparinized tubes at the end of a 28-day
experiment for biochemical investigations.

The activities of AChE and GST decreased at a dose dependent rate (P <0.05). There was no significant difference in the alkaline phosphatase (ALP) of the treated groups and a dose-dependent increase in total protein concentration was detected.

The stem-bark extract did not significantly alter liver biomarkers like ALT, AST, and ALP, particularly at repeated low doses of 50 and 100 mg/kg. The extract of Blighia sapida reduced AChE and GST activity; a property that could be exploited in the formulation of pest control agents.